T cell and cancer cell marker: CHO-131

The monoclonal antibody CHO-131 (mouse IgM) recognizes a specific carbohydrate structure (core-2 O-glycan containing sLeX).  This carbohydrate is a high affinity ligand for the Selectin adhesion molecules (1).  CHO-131 reactivity provides a phenotypic marker for cells that bind to the Selectins (2, 3), which includes human neutrophils and a subset of T cells that migrate to sites of chronic inflammation in the skin, including psoriasis (4).  CHO-131 also detects certain carcinomas and its reactivity correlates with malignant progression and metastasis (5, 6).

1. Walcheck, B., A. Leppanen, R. D. Cummings, R. N. Knibbs, L. M. Stoolman, S. R. Alexander, P. E. Mattila, and R. P. McEver. 2002. The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated with sialyl-Lewis x, which is a functional glycan ligand for P-selectin. Blood 99: 4063–9.

2. Yago, T., A. Leppanen, J. A. Carlyon, M. Akkoyunlu, S. Karmakar, E. Fikrig, R. D. Cummings, and R. P. McEver. 2003. Structurally distinct requirements for binding of P-selectin glycoprotein ligand-1 and sialyl Lewis x to Anaplasma phagocytophilum and P-selectin. J Biol Chem 278: 37987–37997.

3. Smith, M. J., B. R. Smith, M. B. Lawrence, and K. R. Snapp. 2004. Functional analysis of the combined role of the O-linked branching enzyme core 2 beta1-6-N-glucosaminyltransferase and dimerization of P-selectin glycoprotein ligand-1 in rolling on P-selectin. J Biol Chem 279: 21984–21991.

4. Ni, Z., J. J. Campbell, G. Niehans, and B. Walcheck. 2006. The monoclonal antibody CHO-131 identifies a subset of cutaneous lymphocyte-associated antigen T cells enriched in P-selectin-binding cells. J Immunol 177: 4742–4748.

5. St Hill, C. A., K. M. Bullard, and B. Walcheck. 2005. Expression of the high-affinity selectin glycan ligand C2-O-sLeX by colon carcinoma cells. Cancer Lett. 217: 105–113.

6. St Hill, C. A., M. Farooqui, G. Mitcheltree, H. E. Gulbahce, J. Jessurun, Q. Cao, and B. Walcheck. 2009. The high affinity selectin glycan ligand C2-O-sLex and mRNA transcripts of the core 2 beta-1,6-N-acetylglusaminyltransferase (C2GnT1) gene are highly expressed in human colorectal adenocarcinomas. BMC Cancer 9: 79.

7. Chen Z, Gulzar ZG, St. Hill C, Walcheck B, Brooks JD. 2014. Increased expression of GCNT1 is associated with altered O-glycosylation of PSA, PAP and MUC1 in human prostate cancers. Prostrate. 74:1059